Liquid RNase scavenger

Liquid-phase RNase scavenger products and features Liquid-phase RNase scavenger contains special compounds that can inactivate trace amounts of RNase that may be contaminated in solution, suitable for dissolving RNA precipitation. At the same time, it can also be used to treat amino-containing solutions that cannot be treated with DEPC, such as Tris, MOPS, etc. 1. Inactivate RNase in the solution, treat at 60 ° C for 20 minutes to kill 1 ug / mL of RNase, and treat at 37 ° C for 20 minutes to kill 50 ng / mL of RNase. 2. Does not affect most subsequent reactions, including reverse transcription, RNA synthesis and RT-PCR. 3. Effective for RNase A, RNase 1 and RNase T1. 4. Suitable for various buffers, including Tris and MOPS buffers that cannot be treated with DEPC. 5. Easy to use, it can be treated at 60 ℃ for 10-20 minutes. The treated solution can be reprocessed afterwards. After treatment, the solution does not need to be autoclaved (unlike DEPC treatment). 6. This product contains two components, A and B, which can be stored at -20 ℃ for one year, and can be stored at -20 ℃ for six months after being formulated into a solution. Specifications and ingredients Ingredients 1.5 mL Packaging ingredient A 120 mg Ingredient B 1.5 mL user manual 1 copy Transportation and storage Transport at 4 ° C, storage at -20 ° C, valid for 1 year. Self-provided reagent ultrapure water (for diluting this product) How to use 1. Add 120mg of component A to 1.5 mL of component B, shake to dissolve all, get 20X liquid phase RNase scavenger, divide into small packages and put- Long-term storage at 20 ℃. The validity period of 20X liquid RNase scavenger solution is six months. 2. Add 1/20 volume of liquid RNase scavenger to the solution or reaction solution to be treated (eg, 5 uL of liquid RNase scavenger can be added to 100 uL solution). 3. If it is used to dissolve the extracted or purified RNA sample, it is necessary to first dilute the liquid RNase scavenger 20 times with ultrapure water, and then directly used to dissolve RNA (Note: the diluted liquid RNase scavenger cannot be stored for a long time Can only be used now). 4. Treat at 60 ° C for 20 minutes to inactivate contaminated RNase. 5. After cooling to room temperature, it can be used or stored at -20 ℃ for long-term storage. 6. Enzymes that are sensitive to treatment at 60 ° C (such as RNA polymerase) should be added after cooling at 60 ° C. 7. The treated solution can be reprocessed at any time (10-20 minutes at 60 ° C) to remove any possible RNase contamination. 8. If there are samples in the solution that cannot be treated at 60 ° C, they can also be treated at 37 ° C for 20 minutes, but only up to 50 ng / mL of RNase can be inactivated. Related Information DEPC-treated water ≠ RNase-free water Troubleshooting Q: What is better to use this product to dissolve RNA than RNase-free water? A: This product can inactivate RNase remaining in RNA, but RNase-free water cannot. Q: Why can't I use RNase Inhibitor for long-term preservation of RNA? A: RNase Inhibitor is the lowest known RNase A inhibitor of Ki, but it is a protein itself and is easily denatured. In addition, its activity also depends on the reducing agent DTT, and DTT is very easy to inactivate in water (oxygen in water and air), so during long-term storage, RI will gradually inactivate and release the bound RNaseA, but instead make RNA molecules More easily degraded. Q: Will RNA degrade in RNase-free solution? A: In addition to being degraded by RNase, RNA molecules will degrade in the presence of divalent metal ions (such as magnesium ions), or at temperatures above 65 ° C, or in environments with a pH above 9 or below 6. In Tris and boric acid buffers, such reactions occur at neutral pH and normal temperature. Related product solid-phase RNase scavenger (CAT #: 3090) version information from V2.0. The product is divided into A and B components.

1) Easy to clean.