Roche 454 sequencing involved in unraveling banana genome
An international research team led by French researchers reported in Nature that the researchers used a combination of Roche 454 sequencer and other sequencers to complete a sketch of the genome of Musa acuminata. The diploid banana plant contains approximately 5.23 million bases. To further explore the evolutionary history of the banana variety and its relationship with other plants. Musa species and its subspecies have a history of about 7000 years of hybridization, and through the selection and breeding of seedless varieties, diploids and triploids with the genomes of two varieties M. acuminata and M. balbisiana have been developed Banana planting varieties. Researchers are currently focusing on the diploid banana variety called Pahang, which comes from the cross between wild M. acuminata and its subspecies malaccensis. However, wild M. acuminata is also one of the genome components of the triploid banana variety Cavendish, which currently accounts for one-half of all banana production. Using shotgun sequencing data, sequence stitching and genome sketches were drawn, covering about 90% of the Pahang genome. Subsequent analysis predicted 36,542 protein-coding genes and 235 microRNAs. It is also predicted that transposons in the genome account for about half of the sequence in the entire genome. Nearly 7,700 gene clusters in the banana genome shared with other plant lineages, such as Arabidopsis, date palm, rice, sorghum, etc .; at the same time, specific gene clusters were also found, including transcriptional regulators, disease and pest defense Functions related to metabolism. The banana genome sequence also contains plant virus genomes: such as the double-stranded DNA virus banana streak virus. Pahang chromosome analysis confirmed the original understanding of genome replication events, that is, genome-wide replication occurs both in the Musa species and in the flowering plant order (Zingiberales). Finally, RNA sequence data provides some information about the changes in gene transcription that accompany banana fruit ripening. It is well known that the ripening of banana fruits can be induced by the plant hormone gas ethylene. This study found that when bananas ripen, the expression of genes related to the plant cell wall increases rapidly, while the level of amylase decreases. Transcriptome analysis also gives some information about banana defense against pests and diseases, which is one of the most concerned topics in the field of plant plant research. The acquisition of the Musa genome sequence is a major advancement in the complex genome of this important cash crop, laying the foundation for the genetic improvement of this cash crop. The Eyeshadow Brush can fully blend the Eyeliner with the Eye Shadow around the eye and under the eyebrow, so that the layered sense of color can be dulled. The amount of hair is moderate, and the amount of powder makeup is easy to control. The long handle design has excellent stability, which can easily show the delicate lines and let the color spread naturally. Can also be used to shape nose shadows. Crease Brush,Shadow Brush,Eye Makeup Brushes,Eyeshadow Crease Brush Henan Daxin Cosmetics Co., Ltd. , https://www.dx-cosmetics.com