Drug analysis of cefoperazone sulbactam sodium

Cefoperazone sulbactam sodium analysis was performed using a Kromasil C18 column (5 μm, 150 × 4.6 mm). The chromatographic separation was carried out under the following conditions: the mobile phase consisted of 0.005 mol/L tetrabutylammonium hydroxide and acetonitrile in a ratio of 75:25 (v/v). The detection wavelength was set at 220 nm, and the flow rate was maintained at 1.0 mL/min throughout the analysis. This method ensures good resolution and sensitivity for the determination of cefoperazone sulbactam sodium in the sample. The column temperature was kept at ambient, and the injection volume was 20 μL. The system suitability was evaluated by checking the tailing factor and resolution between the peaks. This procedure is suitable for routine quality control testing and provides reliable and reproducible results.

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