Jiangsu Tongjun explains the use and maintenance of biological microscope

First, the correct use of biological microscope

(1) When checking, place the microscope on the front of the seat on the front of the seat, and ask the table to be flat to keep the microscope stable. The mirror base should be about 6-7cm away from the table, and the binocular eyepiece should be as high as the eyes or slightly lower.

(2) After turning on the power, turn on the microscope light source switch to adjust the light intensity to the appropriate size.

(3) Rotate the objective lens converter so that the objective lens is facing the concentrator on the stage. First raise the stage to about 1-2cm from the objective lens, then look into the eyepiece with both eyes, pull the two eyepieces apart or contract until the eyes are coincident to see the same field of view, then adjust the height and aperture of the concentrator The size of the aperture is adjusted to the optimum, so that the light is incident into the lens barrel through the concentrator, and the field of view is bright. If an image of an external object appears in the field of view, the condenser can be lowered slightly and the image can disappear.

(4) Place the slide to be observed on the stage so that the observed part of the slide is located in the center of the light-passing hole, then clip the slide with the specimen holder or pinch the slide with your hand. Observed.

(5) Observing with a microscope of 600 or 640 times (objective lens 15X or 16X, eyepiece 40x). Before the beginner observes, turn the coarse focus hand wheel to raise the stage, and the objective lens gradually approaches the slide and stops. It should be noted that the objective lens cannot be touched by the objective lens to prevent the lens from adhering to the objective lens or the objective lens to crush the slide. Then, look into the eyepiece with both eyes, and turn the coarse focus hand wheel to slowly lower the stage. You can see the magnified image of the sample in the slide soon, and then use the fine adjustment. In order to improve the efficiency of microscopic examination, skilled microscopy personnel can directly look at the eyepiece with both eyes, pinch the slide with the left hand, and turn the coarse focus focus hand wheel with the right hand to raise the magnification of the sample in the slide to the slide. Then, use the fine-motion focus wheel to adjust to a clear picture in the field of view.

(6) If you want to watch several different fields of view, you can slowly adjust the stage moving handle, you can move the stage back and forth or left and right. When adjusting, it should be noted that the direction in which the slide moves is opposite to the direction in which the object image seen in the field of view moves. Skilled microscopyers can move the slides by hand. If the object is not very clear, you can adjust the jog focus wheel until the object is clear.

Second, the maintenance and maintenance of biological microscope

(1) The microscopy personnel must be proficient in the strict use of the procedures. The biological microscope should be used together, and it is best to avoid mixing and arbitrarily disassembling and replacing the parts on the microscope.

(2) When taking the microscope, be sure to hold the curved arm with one hand and the base with the other hand. The microscope should not be tilted to prevent the eyepiece from slipping out of the upper end of the barrel. You cannot move the position of the microscope casually during work.

(3) Where the optical part of the microscope is to be kept clean, it can only be wiped with a soft tissue paper without short fluff. Do not wipe with other items. Keep the mirror paper in the paper tray to prevent dust. Do not rub the lens with a handkerchief or gauze. The objective lens can be cleaned with a solvent if necessary by picking up a small amount of alcohol, acetone or xylene with a defatted cotton swab, and immediately rubbing the alcohol, acetone or xylene with a mirror paper, and then blowing it with an ear wash. Go to the short pile that may remain. Do not touch the lens with your fingers to prevent sweat from smearing the lens.

(d) Whether the eyepiece is clean can be viewed under the microscope. Rotate the eyepiece. If the stain is visible in the field of view, the eyepiece is stained. You can use the lens to wipe the lens. If it can't be removed, wipe the lens below and wipe it off with ear balls. When you wipe the eyepiece or need to remove the eyepiece for other reasons, you should use the lens paper to cover the mouth of the lens barrel to prevent dust from entering the lens barrel and falling on the objective lens under the lens barrel.

(5) The objective lens is the most complicated and important optical component in the microscope. The light is used to image the object to be inspected for the first time. Therefore, the direct relationship and the quality of the imaging and various optical technical parameters are the primary factors for measuring the quality of a microscope. Standard, so it is strictly forbidden to disassemble the objective lens at will. When converting the objective lens or when you need to wipe the objective lens, do not move the animal lens by hand, but use two fingers to turn the converter. Pushing the objective lens directly with your finger is more labor-saving, but it is easy to deflect the optical axis of the objective lens because the material of the converter is soft and the precision is high. The uneven force of the thread is easy to loosen, resulting in low The focus is not aligned when the high magnification objective is switched.

(6) It is not possible to rotate the focusing hand wheel at random. When using the jog focus knob, the force should be light, the rotation should be slow, and the hard turn should not be hard.

(7) When using a high magnification objective lens, unskilled microscopy personnel should not adjust the focal length with the coarse focus hand wheel to avoid excessive movement distance and damage the objective lens and slide.

(8) Pay attention to the environmental hygiene of the microscopic examination, keep it dry and clean, pay attention to dust and mildew. Dust and mold are the "big enemies" of the microscope, the dust is pervasive, and the mold spores are everywhere. Therefore, a desiccant should be placed in the microscope case.

(9) Keep the microscope clean and prevent contamination of water and chemical reagents. In particular, the microscope used in the inspection of silkworm samples has strong alkaline liquid and strong acidic liquid (such as KOH, NaHCO3, HCI, bleaching powder, etc.) in the work site, which is very corrosive and easily causes rusting of the microscope. phenomenon.

(10) After using it every day, you must check whether the lens is stained with water or dirt. If it is, wipe it clean and wipe the stage clean. After each use phase, wipe it once, especially the eyepiece and objective lens. At the same time, add some sewing machine oil as lubricant to each active part, lower the mirror body to the lowest position, adjust the position of the specimen mover on the stage, and then adjust the position of the specimen mover on the stage. Place the microscope in a desiccant box and pay attention to the safety box.

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