Shanghai Xinfan Bio offers the Human Basic Fibroblast Growth Factor (bFGF) ELISA Kit, designed for accurate and reliable detection of bFGF levels in various biological samples. We provide free testing services, along with detailed experimental reports and raw data analysis, all available for one week after the test. Our professional team is here to support you throughout the entire process. Contact us today to learn more about our services and how we can assist your research.
As a reputable supplier of high-quality ELISA kits, Shanghai Xinfan Bio has built a strong market reputation through its dedicated sales and technical support teams. We are an authorized agent for enzyme-free reagents, ensuring that our products meet the highest standards of quality and performance. Our direct delivery service ensures convenience, and we are always ready to help you with any inquiries or assistance you may need.
In addition to selling ELISA kits, we also offer comprehensive testing services. You can purchase our kits at no cost for initial testing, and our experienced scientists will handle the experiments. With access to authoritative enzyme-free laboratories, we guarantee accurate results, detailed reports, and reliable data—all within a week. This helps you save time and reduces the risk of errors in your experiments.
**Sample Collection Guidelines:**
Before collecting samples, it's essential to have a clear plan and ensure that the target analytes remain stable. For samples tested on the same day, store them at 4°C immediately. If samples are to be tested every other day, they should be stored at -20°C after proper aliquoting. For long-term storage, freezing at -70°C is recommended. Avoid repeated freeze-thaw cycles to maintain sample integrity.
**Sample Preparation Procedures:**
1. **Serum:** Allow blood to clot naturally at room temperature for 10–20 minutes, then centrifuge at 2000–3000 rpm for 20 minutes. Carefully collect the supernatant. If precipitation occurs during storage, re-centrifuge before use.
2. **Plasma:** Use anticoagulants such as EDTA, sodium citrate, or heparin, depending on the requirements. Mix for 10–20 minutes, then centrifuge at 2000–3000 rpm for 20 minutes. Collect the supernatant carefully, and re-centrifuge if needed.
3. **Urine:** Collect using a sterile container, then centrifuge at 2000–3000 rpm for 20 minutes. Carefully collect the supernatant. The same method applies to pleural fluid, ascites, and cerebrospinal fluid.
4. **Cell Culture Supernatant:** Collect the supernatant in a sterile tube, then centrifuge at 2000–3000 rpm for 20 minutes. Carefully collect the supernatant for further analysis.
5. **Cell Lysate:** Suspend cells in PBS (pH 7.2–7.4) at a concentration of approximately 1 million cells/mL. Lyse the cells by repeated freezing and thawing or by adding a tissue protein extraction reagent. Centrifuge at 2000–3000 rpm for 20 minutes and collect the supernatant. Re-centrifuge if necessary.
6. **Tissue Samples:** Weigh the tissue, add PBS (pH 7.4), and rapidly freeze in liquid nitrogen. After thawing, maintain the sample at 2–8°C. Add PBS or tissue protein extraction reagent, homogenize using a manual or mechanical homogenizer, then centrifuge at 2000–3000 rpm for 20 minutes. Use part of the supernatant for testing and store the rest for future use.
7. **General Tips:** Extract samples as soon as possible after collection and follow appropriate protocols from relevant literature. Perform the experiment promptly after extraction. If not testing immediately, store the samples at -20°C, avoiding repeated freeze-thaw cycles.
8. **Note:** Samples containing NaN3 cannot be used for ELISA testing, as NaN3 inhibits horseradish peroxidase (HRP) activity, which is essential for the detection process.
Our goal is to provide you with high-quality ELISA solutions, expert support, and reliable results. Let us help you achieve your research goals efficiently and effectively. Contact us now for more information!
Biodegradable plates are an eco-friendly alternative to traditional disposable plates, which are often made from non-biodegradable materials like plastic or Styrofoam. When these plates are discarded, they can take hundreds of years to break down in landfills and can contribute to pollution and environmental damage.
Biodegradable Plate,Biodegradable Cpla Plates,Cornstarch Cpla Plates,Disposable Cornstarch Cpla Plates
Anhui Jianfeng Environmental Protection Technology Co., Ltd , https://www.ahbiocutleries.com