Shanghai Xinfan Bio offers a high-quality Human Basic Fibroblast Growth Factor (bFGF) ELISA Kit, designed for accurate and reliable detection of bFGF in various biological samples. We provide free testing services, along with detailed experimental reports and raw data analysis, all available for one week after the test. Our team is committed to delivering professional support, ensuring that your research is both efficient and precise. Whether you're conducting basic research or clinical studies, we are here to assist you every step of the way.
As a reputable supplier in the life science industry, Shanghai Xinfan Bio has built a strong market reputation, supported by a dedicated sales and technical team. We are an authorized enzyme-free agent, offering expert guidance and assistance throughout the entire testing process. Our ELISA kits are delivered directly to your doorstep, making it easier for you to access high-performance reagents without unnecessary delays. Contact us today to learn more about our products and services.
In addition to selling ELISA kits, we also provide comprehensive testing services. You can purchase our kits at no cost, and our authoritative enzyme-free laboratory will conduct the tests using experienced scientists. The results, including detailed reports and raw data, will be provided within a week, ensuring the accuracy and reliability of your findings. This helps you save time and reduces the risk of experimental errors, allowing you to focus on your research goals.
**Sample Collection Guidelines:**
Before collecting any sample, it's essential to have a clear plan and ensure that the target analytes are stable. For samples tested on the same day, store them at 4°C immediately. If retesting is required every other day, store them at -20°C after proper aliquoting. For long-term storage, freezing at -70°C is recommended. Avoid repeated freeze-thaw cycles to maintain sample integrity.
- **Serum:** Allow blood to clot at room temperature for 10–20 minutes, then centrifuge at 2000–3000 rpm for 20 minutes. Carefully collect the supernatant. If precipitation occurs, re-centrifuge before use.
- **Plasma:** Use anticoagulants such as EDTA, sodium citrate, or heparin. Mix thoroughly for 10–20 minutes, then centrifuge at 2000–3000 rpm for 20 minutes. Collect the supernatant carefully and re-centrifuge if needed.
- **Urine:** Collect in a sterile container and centrifuge at 2000–3000 rpm for 20 minutes. Carefully collect the supernatant. Repeat centrifugation if precipitate forms during storage.
- **Cell Culture Supernatant:** Collect in a sterile tube, centrifuge at 2000–3000 rpm for 20 minutes, and carefully collect the supernatant.
- **Cell Lysate:** Dilute the cell suspension in PBS (pH 7.2–7.4) to approximately 1 million cells/mL. Lyse the cells through repeated freeze-thaw cycles or by adding tissue protein extraction reagent. Centrifuge at 2000–3000 rpm for 20 minutes and collect the supernatant.
- **Tissue Specimens:** Weigh the tissue, add PBS (pH 7.4), and quickly freeze in liquid nitrogen. After thawing, homogenize the tissue using a manual or mechanical homogenizer, then centrifuge at 2000–3000 rpm for 20 minutes. Store a portion for testing and freeze the rest for future use.
- **General Tips:** Extract samples as soon as possible after collection and follow standard protocols. If testing cannot be done immediately, store samples at -20°C, avoiding repeated freeze-thaw cycles.
- **Note:** Samples containing NaN3 should not be used, as this compound inhibits horseradish peroxidase (HRP), which is commonly used in ELISA assays.
Let us help you achieve accurate and reproducible results. Contact us now to discuss your needs and take advantage of our full-service ELISA solutions.
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