Human P-selectin (PS) enzyme-linked immunosorbent assay (ELISA) kit instruction manual
Deep Wave Wig Transparent 13x4 13x6 Hd,Deep Wave Wig Hd Lace,Deep Wave Frontal Wig Human Hair Xu Chang Zhuo YunQing crafts Co., LTD , https://www.wowqueenhair.com
This reagent is intended for research use only. The kit is designed to quantify the level of P-selectin (PS) in human serum, plasma, and other related biological samples. Principle of the assay: The double-antibody sandwich ELISA method is employed to detect human P-selectin. A microplate is pre-coated with a purified monoclonal antibody specific to P-selectin. After incubation with the sample, the P-selectin present in the sample binds to the immobilized antibody. Then, an HRP-conjugated secondary antibody is added, forming a complex of antibody-antigen-enzyme-labeled antibody. Following thorough washing, TMB substrate is introduced. Under the catalytic action of HRP, TMB turns blue, and upon addition of an acidic stop solution, it changes to yellow. The intensity of the color is directly proportional to the concentration of P-selectin in the sample. The optical density (OD) is measured at 450 nm using a microplate reader, and the concentration of P-selectin in the sample is determined by comparing the OD value with a standard curve.
Kit components include: 1 × 48-well or 1 × 96-well plate, 2 sealing films, 1 sealed bag, 1 enzyme-labeled plate, 1 standard (36 ng/L), 1 standard diluent, 1 enzyme reagent, 1 sample diluent, 1 developer A, 1 developer B, 1 stop solution, and a concentrated wash solution (20 ml × 20 times or 20 ml × 30 times). All reagents should be stored at 2–8°C. The kit is suitable for quantitative analysis of P-selectin in clinical and research settings, ensuring high specificity and sensitivity. It is recommended to follow the manufacturer’s instructions carefully to ensure accurate and reproducible results.