Duck Casp-3 ELISA Kit

**Duck Casp-3 ELISA Kit – For the Quantitative In Vitro Determination of Duck B-cell Leukemia/Lymphoma 2 Concentrations in Serum, Plasma, Cerebrospinal Fluid, Tissue Homogenate, and Body Fluids** *For Laboratory Research Use Only. Not for Diagnostic or Therapeutic Procedures.* Before using this product, please read this entire package insert carefully. This Duck Casp-3 ELISA Kit is designed for research purposes only and should not be used in diagnostic or clinical settings. The assay is based on the principle of enzyme-linked immunosorbent assay (ELISA), where the color change from blue to yellow is induced by the Stop Solution, and the intensity of the color is measured at 450 nm using a spectrophotometer. To determine the concentration of Casp-3 in your samples, this kit includes a set of calibration standards. By plotting the optical density (OD) values against known concentrations, you can generate a standard curve. The Casp-3 concentration in unknown samples is then calculated by comparing their OD values to this curve. **Sample Collection and Storage** - **Serum**: Use a serum separator tube. Allow samples to clot for 2 hours at room temperature or overnight at 4°C before centrifugation at 2000×g for 20 minutes. Remove serum and assay immediately, or aliquot and store at -20°C. Avoid repeated freeze-thaw cycles. - **Plasma**: Collect using heparin as an anticoagulant. Centrifuge within 30 minutes at 2000×g, 2–8°C. Store at -20°C. - **Cell Culture Supernatants, Tissue Homogenates, and Other Biological Fluids**: Centrifuge to remove particulates and assay immediately or store at -20°C. Avoid repeated freezing. *Note: Ensure adequate centrifugation and avoid hemolysis or granulation in samples.* **Materials Required but Not Supplied** 1. 37°C incubator 2. Microplate reader capable of measuring absorbance at 450 nm 3. Precision pipettes, disposable tips, and absorbent paper 4. Distilled or deionized water **Reagents Provided** All reagents are stored at 2–8°C. Check the expiration date on the label. | Reagent | 96 Determinations | 48 Determinations | |--------|-------------------|-------------------| | MicroELISA Strip Plate | 12×8 strips | 12×4 strips | | Standard (6 vials) | 0.5 ml/vial | 0.5 ml/vial | | Sample Diluent | 6.0 ml | 3.0 ml | | HRP-Conjugate Reagent | 10.0 ml | 5.0 ml | | 20X Wash Solution | 25 ml | 15 ml | | Chromogen Solution A | 6.0 ml | 3.0 ml | | Chromogen Solution B | 6.0 ml | 3.0 ml | | Stop Solution | 6.0 ml | 3.0 ml | | Closure Plate Membrane | 2 | 2 | | User Manual | 1 | 1 | | Sealed Bags | 1 | 1 | *Note: Standard concentrations are 200, 100, 50, 25, 12.5, 6.25 pmol/L. If sample values exceed the highest standard, dilute with Sample Diluent and repeat the assay.* **Precautions** 1. Do not mix reagents from different kit lots. 2. All reagents must be brought to room temperature (20–25°C) before use. 3. Never use expired reagents. 4. Use only deionized or distilled water for dilution. 5. Keep microtiter plates in sealed bags until use. 6. Always use fresh pipette tips to prevent contamination. 7. Handle all biological samples with care. Wear gloves and follow biosafety protocols. 8. Dispose of waste properly after inactivation for at least 30 minutes. 9. Substrate solutions must be handled carefully—avoid exposure to light and heat. 10. Allow all reagents to reach room temperature before starting the assay. **Reagent Preparation and Storage** - **Wash Solution (1X)**: Dilute 1 volume of 20X Wash Solution with 19 volumes of deionized or distilled water. Store at 2–8°C for up to one month. **Assay Procedure** 1. Prepare all reagents before starting. Run standards and samples in duplicate. 2. Add 50 µl of standard or sample to each well. Blank well receives no addition. 3. Add 100 µl of HRP-Conjugate Reagent to all wells except the blank. Cover with adhesive strip and incubate for 60 minutes at 37°C. 4. Wash the plate 4 times. Manual washing: Fill each well with 1X Wash Solution, aspirate, and repeat. Automated washing: Use appropriate settings. 5. Add 50 µl of Chromogen A and 50 µl of Chromogen B to each well. Incubate for 15 minutes at 37°C, protected from light. 6. Add 50 µl of Stop Solution to each well. Color changes from blue to yellow. If green or uneven color appears, gently tap the plate. 7. Measure OD at 450 nm. Generate a standard curve by plotting OD vs. concentration. Calculate sample concentrations accordingly. **Performance Characteristics** - Intra-assay and inter-assay CV% < 15%. - Assay range: 6.25 pmol/L – 200 pmol/L. - Sensitivity: < 1.0 pmol/L. - Cross-reactivity: No significant cross-reaction observed. - Storage: 2–8°C (frequent use); -20°C for up to six months. This kit is ideal for researchers studying apoptosis-related proteins in avian models. Always follow safety guidelines and ensure proper handling of all reagents and samples.

Dining Chair

Dining chairs are more than just a functional necessity; they’re an integral part of your dining room’s aesthetic and ambiance. A thoughtfully chosen dining chair can transform a dining area, creating a welcoming space where family and friends come together to enjoy meals and conversations. Our collection of dining chairs includes a variety of styles, materials, and finishes, allowing you to find the perfect chairs to match your table and reflect your personal taste.
Our dining chair selection ranges from modern, sleek designs to timeless classics, making it easy to find a style that complements any decor. For those who love minimalist elegance, our modern dining chairs feature clean lines, neutral tones, and streamlined frames crafted from materials like metal, wood, and leather. These chairs emphasize simplicity and functionality, blending effortlessly into contemporary dining spaces.
Classic dining chairs offer timeless appeal and sophistication, with details like high backs, carved wood accents, and plush upholstery. These traditional designs provide a refined look that’s perfect for formal dining settings or spaces with a classic or vintage feel. Many of our classic chairs are crafted from solid wood and feature luxurious upholstery options, including soft fabrics and leather, for a touch of elegance and comfort.
Comfort is key in any dining chair, and we offer various options to suit different needs. Upholstered dining chairs are a popular choice, providing extra padding and support that make long meals and gatherings more enjoyable. These chairs come in a variety of fabric choices, including linen, velvet, and leather, each adding its own texture and feel to the room. For added back support, high-back dining chairs create an ergonomic seating option that combines style and functionality. Additionally, many of our chairs have curved or padded armrests, offering even more comfort for extended dining experiences.
When it comes to materials, our dining chairs are crafted with durability and style in mind. Solid wood chairs offer a sturdy and classic option, available in finishes like natural oak, walnut, and painted wood, ensuring they match both rustic and modern interiors. Metal dining chairs, often finished in sleek black or chrome, offer a contemporary look and exceptional durability. These materials are easy to maintain and perfect for households with young children or frequent dinner guests.
Our dining chairs are designed to withstand daily use while maintaining their visual appeal. Each chair is crafted with high-quality materials and goes through rigorous quality checks to ensure it meets our standards for both style and functionality. This means you can enjoy both the aesthetic and practical benefits of our dining chairs for years to come.
Choosing the right dining chairs allows you to personalize your dining space, creating an atmosphere that aligns with your lifestyle and taste. Whether you’re seeking cozy, upholstered chairs for a more intimate feel or streamlined modern chairs for a minimalist vibe, our range offers a variety of options to suit every home. With a focus on quality, comfort, and design, our dining chairs are the perfect addition to any dining room, enhancing both everyday meals and special gatherings.

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Foshan Shunde Baotian Furniture Co., Ltd. , http://www.baotian.com